silicic acid hplc column (Waters Corporation)
Waters Corporation manufactures this product 
86
Structured Review
Waters Corporation
silicic acid hplc column
Silicic Acid Hplc Column, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/silicic+acid+hplc+column/pmc03783530-64-1-19
Average 86 stars, based on 1 article reviews
Silicic Acid Hplc Column, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/silicic+acid+hplc+column/pmc03783530-64-1-19
Average 86 stars, based on 1 article reviews
silicic acid hplc column - by Bioz Stars,
2026-10
86/100 stars
Images
1) Product Images from "Dual Effect of Heparin on Fe 2+ Induced Cardiolipin Peroxidation. Implications to Peroxidation of Cytochrome c Oxidase Bound Cardiolipin ‡ "
Article Title: Dual Effect of Heparin on Fe 2+ Induced Cardiolipin Peroxidation. Implications to Peroxidation of Cytochrome c Oxidase Bound Cardiolipin
Journal: Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry
doi: 10.1007/s00775-013-1019-z
Figure Legend Snippet: Upper panel – silicic acid HPLC chromatogram. The numbers (I, II and III) designate the randomly chosen areas where spectra were analyzed. Lower panels ( b, c and d ) represent spectra extracted from the randomly chosen areas (I, II and III) of silicic acid HPLC. Solid line is CL extracted from 5 μM CcO; dotted line is CL extracted from 5 μM CcO incubated with 5 mM tert-BOOH and 50 μM Fe2+; dashed line is CL extracted from 5 μM CcO incubated with 5 mM tert-BOOH, 50 μM Fe2+ and 100 μM heparin.
Techniques Used: Incubation
Figure Legend Snippet: CcO (5μM) solubilized in 50 mM Tris-SO4 buffer, pH 7.4, containing 2 mM dodecyl maltoside and 2 mM sodium cholate was incubated for 60 min at room temperature with: (1) no additions; (2) 5 mM tert-BOOH ± 100 μM heparin ; (3) 5 mM tert-BOOH + 50 μM Fe2+; (4) 5 mM tert-BOOH + 50 μM Fe2+ + 100 μM heparin; (5) 50 μM Fe2+ ; (6) CcO + 50 μM Fe2+ + 100 μM heparin. The reaction was stopped by dilution and the activity measured spectrophotometrically with reduced cytochrome c as a substrate. These results are representative of three independent experiments. The error in determination of molecular activity was ±2.6%.
Techniques Used: Incubation, Activity Assay
Related Articles
High Performance Liquid Chromatography:Article Title: Dual Effect of Heparin on Fe 2+ Induced Cardiolipin Peroxidation. Implications to Peroxidation of Cytochrome c Oxidase Bound Cardiolipin Article Snippet: .. The Article Title: Phospholipase A(2) digestion of cardiolipin bound to bovine cytochrome c oxidase alters both activity and quaternary structure. Article Snippet: Phospholipase A2 from Crotalus atrox hydrolyzes all of the phospholipids that are associated with purified, detergent-solubilized cytochrome c oxidase; less than 0.05 mol cardiolipin (CL)1 remains bound per mol enzyme.. Coincident with the hydrolysis of cardiolipin is a reversible decrease of 45-50% in the electron transport activity of the dodecylmaltoside-solubilized enzyme.. Full activity is recoverable (90-98%) by addition of exogenous cardiolipin, but not by either phosphatidylcholine or phosphatidylethanolamine. Fast Protein Liquid Chromatography:Article Title: Phospholipase A(2) digestion of cardiolipin bound to bovine cytochrome c oxidase alters both activity and quaternary structure. Article Snippet: Phospholipase A2 from Crotalus atrox hydrolyzes all of the phospholipids that are associated with purified, detergent-solubilized cytochrome c oxidase; less than 0.05 mol cardiolipin (CL)1 remains bound per mol enzyme.. Coincident with the hydrolysis of cardiolipin is a reversible decrease of 45-50% in the electron transport activity of the dodecylmaltoside-solubilized enzyme.. Full activity is recoverable (90-98%) by addition of exogenous cardiolipin, but not by either phosphatidylcholine or phosphatidylethanolamine. |